Automated Home-Cage Functional Imaging

Timothy Murphy and his colleagues at the University of British Columbia have developed an automated system for mesoscopic functional imaging that allows subjects to self-initiate head-fixation and imaging within the home-cage. In their 2016 paper, “High-throughput automated home-cage mesoscopic functional imaging of mouse cortex,” Dr. Murphy and his colleagues present this device and demonstrate its use with a group of calcium indicator transgenic mice. The supplementary material to this paper includes a diagram of the hardware, a graphic representation of the training cage, several videos of subjects interacting with the device and sample imaging data. The Python source code and 3D print files can be found on Dr. Murphey’s UBC webpage.

Murphy, T. H., Boyd, J. D., Bolaños, F., Vanni, M. P., Silasi, G., Haupt, D., & LeDue, J. M. (2016). High-throughput automated home-cage mesoscopic functional imaging of mouse cortex. Nature Communications, 7, 11611.

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