Category: Behavioral Apparatus

VASIC

September 19, 2018

In HardwareX, Brendan Drackley and colleagues share VASIC, an open source weight-bearing device for high-throughput and unbiased behavioral pain assessment in rodents.


The assessment of pain in animal models is a key component in understanding and developing treatments for chronic pain. Drackley and colleagues developed VASIC (Voluntary Access Static Incapacitance Chamber), a modified version of a weight-bearing test. A brief water deprivation encourages rats or mice to seek water in a test chamber, set up with a weighing platforms under the water spout, which can assess weight shifting to an unaffected side in animals with damage to nerves or inflammatory pain. The design incorporates a custom printed circuit board (available from the paper), infrared sensor, Arduino microcontroller, 3D printed parts, and open source software for analysis. A full parts list, links to files, and data from a validation study are available in their paper.

Read more here!


An inexpensive, scalable Picamera system for tracking rats in large spaces

August 15, 2018

In the Journal of Neurophysiology, Sachin S. Deshmuhk and colleagues share their design for a Picamera system that allows for tracking of animals in large behavioral arenas.


Studies of spatial navigation and its neural correlates have been limited in the past by the reach of recording cables and tracking ability in small behavioral arenas. With the implementation of long-range, wireless neural recording systems, researchers are not able to expand the size of their behavioral arenas to study spatial navigation, but a way to accurately track animals in these larger arenas is necessary. The Picamera system is a low-cost, open-source scalable multi-camera tracking system that can be used to track behavior in combination with wireless recording systems. The design is comprised of 8 overhead Raspberry Pi cameras (capable of recording at a high frame rate in a large field of view) recording video independently in individual Raspberry Pi microcomputers and processed using the Picamera Python library. When compared with a commercial tracking software for the same purpose, the Picamera system reportedly performed better with improvements in inter-frame interval jitter and temporal accuracy, which improved the ability to establish relationships between recorded neural activity and video. The Picamera system is an affordable, efficient solution for tracking animals in large spaces.

Read more here!

Or check out their GitHub!


Saxena, R., Barde, W., and Deshmukh, S.S. An inexpensive, scalable camera system for tracking rats in large spaces (2018). Journal of Neurophysiology. https://doi.org/10.1152/jn.00215.2018

An opensource lickometer and microstructure analysis program

August 8, 2018

In HardwareX, an open access journal for designing, building and customizing opensource scientific hardware, Martin A. Raymond and colleagues share their design for a user-constructed, low-cost lickometer.


Researchers interested in ingestive behaviors of rodents commonly use licking behavior as a readout for the amount of fluid a subject consumes, as recorded by a lickometer. Commercially available lickometers are powerful tools to measure this behavior, but can be expensive and often require further customization. The authors offer their own design for an opensource lickometer that utilizes readily available or customizable components such as a PC sound card and 3D printed drinking bottle holder. The data from this device is collected by Audacity, and opensource audio program, which is then converted to a .csv format which can be analyzed using an R script made available by the authors to assess various features of licking microstructure. A full bill of materials, instructions for assembly and links to design files are available in the paper.

Check out the full publication here!


Raymond, M. A., Mast, T. G., & Breza, J. M. (2018). An open-source lickometer and microstructure analysis program. HardwareX, 4. doi:10.1016/j.ohx.2018.e00035

Collaboration between OpenBehavior and Hackaday.io

July 23, 2018

OpenBehavior has been covering open-source neuroscience projects for a few years, and we are always thrilled to see projects that are well documented and can be easily reproduced by others.  To further this goal, we have formed a collaboration with Hackaday.io, who have provided a home for OpenBehavior on their site.  This can be found at: https://hackaday.io/OpenBehavior, where we currently have 36 projects listed ranging from electrophysiology to robotics to behavior.  We are excited about this collaboration because it provides a straightforward way for people to document their projects with instructions, videos, images, data, etc.  Check it out, see what’s there, and if you want your project linked to the OpenBehavior page simply tag it as “OPENBEHAVIOR” or drop us a line at the Hackaday page.

Note: This collaboration between OpenBehavior and Hackaday.io is completely non-commercial, meaning that we don’t pay Hackaday.io for anything, nor do we receive any payments from them.  It’s simply a way to further our goal of promoting open-source neuroscience tools and their goal of growing their science and engineering community.


https://hackaday.io/OpenBehavior

 

Microwave-based Homecage Motion Detector

June 25, 2018

Andreas Genewsky and colleagues from the Max Planck Institute of Psychiatry have shared the design, construction and validation of a simplified, low-cost, radar-based motion detector for home cage activity monitoring in mice. This simple, open-source device allows for motion detection without visual contact to the animal and can be used with various cage types. It features a custom printed circuit board and motion detector shield for Arduino, which saves raw activity and timestamped data in CSV files onto an SD card; the authors also provide a Python script for data analysis and generation of actograms. This device offers a cost-effective, DIY alternative to optical imaging of home-cage activity.

Read more from the Journal of Biomedical Engineering publication!


Genewsky, A., Heinz, D. E., Kaplick, P. M., Kilonzo, K., & Wotjak, C. T. (2017). A simplified microwave-based motion detector for home cage activity monitoring in mice. Journal of Biological Engineering,11(1). doi:10.1186/s13036-017-0079-y

Open source modules for tracking animal behavior and closed-loop stimulation based on Open Ephys and Bonsai

June 15, 2018

In a recent preprint on BioRxiv, Alessio Buccino and colleagues from the University of Oslo provide a step-by-step guide for setting up an open source, low cost, and adaptable system for combined behavioral tracking, electrophysiology, and closed-loop stimulation. Their setup integrates Bonsai and Open Ephys with multiple modules they have developed for robust real-time tracking and behavior-based closed-loop stimulation. In the preprint, they describe using the system to record place cell activity in the hippocampus and medial entorhinal cortex, and present a case where they used the system for closed-loop optogenetic stimulation of grid cells in the entorhinal cortex as examples of what the system is capable of. Expanding the Open Ephys system to include animal tracking and behavior-based closed-loop stimulation extends the availability of high-quality, low-cost experimental setup within standardized data formats.

Read more on BioRxiv, or on GitHub!


Buccino A, Lepperød M, Dragly S, Häfliger P, Fyhn M, Hafting T (2018). Open Source Modules for Tracking Animal Behavior and Closed-loop Stimulation Based on Open Ephys and Bonsai. BioRxiv. http://dx.doi.org/10.1101/340141

Head-Fixed Setup for Combined Behavior, Electrophysiology, and Optogenetics

June 12, 2018

In a recent publication in the Frontiers in Systems Neuroscience, Solari and colleagues of the Hungarian Academy of Sciences and Semmelweis University have shared the following about a behavioral setup for temporally controlled rodent behavior. This arrangement allows for training of head-fixed animals with calibrated sound stimuli, precisely timed fluid and air puff presentations as reinforcers. It combines microcontroller-based behavior control with a sound delivery system for acoustic stimuli, fast solenoid valves for reinforcement delivery and a custom-built sound attenuated chamber, and is shown to be suitable for combined behavior, electrophysiology and optogenetics experiments. This system utilizes an optimal open source setup of both hardware and software through using Bonsai, Bpod and OpenEphys.

Read more here!

GitHub


Solari N, Sviatkó K, Laszlovszky T, Hegedüs P and Hangya B (2018). Open Source Tools for Temporally Controlled Rodent Behavior Suitable for Electrophysiology and Optogenetic Manipulations. Front. Syst. Neurosci. 12:18. doi: 10.3389/fnsys.2018.00018

Automated mouse homecage two-bottle choice test

May 21, 2018

Meaghan Creed has developed a novel device for assessing preferences by mice among fluids in their homecages, i.e. two-bottle choice test. She shared the design on http://hackaday.io and contributed the summary of it below.

https://hackaday.io/project/158279-automated-mouse-homecage-two-bottle-choice-test

Often in behavioral neuroscience, we need to measure how often and how much a mouse will consume multiple liquids in their home cage. Examples include sucrose preference tasks in models of depression, or oral drug self-administration (ie. Morphine, opiates) in the context of addiction. Classically, two bottles are filled with liquids and volumes are manually recorded at a single time point. Here, we present a low-cost, two-sipper apparatus that mounts on the inside of a standard mouse cage. Interactions are detected using photointerrupters at the base of each sipper which are logged to an SD card using a standard Arduino. Sippers are constructed from 15 mL conical tubes which allows additional volumetric measurements, the rest of the holding apparatus is 3D printed, and the apparatus is constructed with parts from Arduino and Sparkfun. This automated approach allows for high temporal resolution collected over 24 hours, allowing measurements of patterns of intake in addition to volume measurements. Since we don’t need to manually weigh bottles we can do high-throughput studies, letting us run much larger cohorts.

This is designed such that each set of 2 sippers uses its own Arduino and SD card. With a bit of modification to the code one Arduino Uno can be programmed to log from 6 cages onto the same SD card. Arduino compatible boards with more GPIOs (like Arduino Mega) can log from up to 56 sippers on one Arduino.

Open-source touch-screen for rodent behavioral testing

March 9, 2018

O’Leary and colleagues describe an open-source touch-screen for rodent behavioral testing. The manuscript is well documented and includes all of the parts needed to build the system on your own. Very useful methods for testing cognitive function and relating findings across species (rodents, primates, humans). Congrats to the authors on setting a high standard for open-source neuroscience!

O’Leary, J.D., O’Leary, O.F., Cryan, J.F. et al. Behav Res (2018). https://doi.org/10.3758/s13428-018-1030-y

SnackClock

March 1, 2018

From the Kravitz lab at the NIH comes a simple device for dispensing pre-measured quantities of food at regular intervals throughout the day.  Affectionately known as “SnackClock”, this device uses a 24-hour clock movement to rotate a dispenser wheel one revolution per day.  The wheel contains 12 compartments, which allows the device to dispense 12 pre-measured “snacks” at regular 2 hour intervals.  The Kravitz lab has used this device to dispense high-fat diet throughout the day, rather than giving mice one big piece once per day.  The device is very simple to build and use, requiring just two 3D printed parts and a ~$10 clock movement.  There is no microcontroller or coding required for this device, and it runs on one AA battery for >1 year.  The 3D files are supplied and can be edited to fit SnackClock in different brands of caging, or to adjust the number of snack compartments.  With additional effort the clock movement could be replaced by a stepper motor to allow for dispensing at irregular or less frequent intervals.

https://kravitzlab.github.io/SnackClock