May 15, 2018
We developed a toolbox for videographic processing of head-fixed rodent behaviors. It extracts the principal components of the animal’s behavior, either in a single movie, or across movies recorded simultaneously. Several regions of interest in the movie can be processed simultaneously (such as the whisker pad or the nose). We found that the behavioral components from the full face of the mouse predicted up to half of the explainable neural activity across the brain (see https://www.biorxiv.org/content/early/2018/04/22/306019).
In addition to extracting movement components, it can compute the pupil area of the rodent using a center-of-mass estimation. Also, in experiments in which the mouse sits on a surface with texture, the software can estimate the running speed of the mouse. The software is available here (https://github.com/carsen-stringer/FaceMap).
April 2, 2018
Check out the Ethoscopes platform!
Ethoscopes enable high-throughput analysis of behavior in Drosophila and other animals for <$100. The system is capable of real-time video tracking, is based on raspberry pi, and even has its own R package for data analysis. All software and build specifications are available at http://lab.gilest.ro/ethoscope.
An interesting summary of recent methods for monitoring behavior in rodents was published this week in Nature.The article mentions Lex Kravitz and his lab’s efforts on the Feeding Experimentation Device (FED) and also OpenBehavior. Check it out: https://www.nature.com/articles/d41586-018-02403-5
January 8th, 2018
The de Bivort lab and FlySorter, LLC are happy to share on OpenBehavior their open-source Drosophila handling platform, called MAPLE: Modular Automated Platform for Large-Scale Experiments.
Drosophila Melanogaster has proven a valuable genetic model organism due to the species’ rapid reproduction, low-maintenance, and extensive genetic documentation. However, the tedious chore of handling and manually phenotyping remains a limitation with regards to data collection. MAPLE: a Modular Automated Platform for Large-Scale Experiments provides a solution to this limitation.
MAPLE is a Drosophila-handing robot that boasts a modular design, allowing the platform to both automate diverse phenotyping assays and aid with lab chores (e.g., collecting virgin female flies). MAPLE permits a small-part manipulator, a USB digital camera, and a fly manipulator to work simultaneously over a platform of flies. Failsafe mechanisms allow users to leave MAPLE unattended without risking damage to MAPLE or the modules.
The physical platform integrates phenotyping and animal husbandry to allow end-to-end experimental protocols. MAPLE features a large, physically-open workspace for user convenience. The sides, top, and bottom are made of clear acrylic to allow optical phenotyping at all time points other than when the end-effector carriages are above the modules. Finally, the low cost and scalability allow large-scale experiments ($3500 vs hundreds of thousands for a “fly-flipping” robot).
MAPLE’s utility and versatility were demonstrated through the execution of two tasks: collection of virgin female flies, and a large-scale longitudinal measurement of fly social networks and behavior.
Links to materials:
Raw data and analysis scripts
De Bivort Lab Site
January 3rd, 2018
The following behavioral platform was developed and published by Xinfeng Chen and Haohong Li, from Huazhong University of Science and Technology, Wuhan, China
ArControl: Arduino Control Platform is a comprehensive behavioral platform developed to deliver stimuli and monitor responses. This easy-to-use, high-performance system uses an Arduino UNO board and a simple drive circuit along with a stand-along GUI application. Experimental data is automatically recorded with the built-in data acquisition function and the entire behavioral schedule is stored within the Arduino chip. Collectively, this makes ArControl a “genuine, real-time system with high temporal resolution”. Chen and Li have tested ArControl using a Go/No-Go task and a probabilistic switching behavior task. The results of their work show that ArControl is a reliable system for behavioral research.
Source codes and PCB drafts may be found here: ArControl Github
December 18, 2017
ZebraTrack is a cost-effective imaging setup for distraction-free behavioral acquisition with automated tracking using open-source ImageJ software and workflow for extraction of behavioral endpoints of zebrafish. This ImageJ algorithm is capable of providing control to users at key steps while maintaining automation in tracking without the need for the installation of external plugins.
Nema, S., Hasan, W., Bhargava, A., & Bhargava, Y. (2016). A novel method for automated tracking and quantification of adult zebrafish behaviour during anxiety. Journal of Neuroscience Methods, 271, 65-75. doi:10.1016/j.jneumeth.2016.07.004
November 28, 2017
Pyper is developed by The Margrie Laboratory.
Pyper provides real-time or pre-recorded motion tracking of a specimen in an open-field. Pyper can send TTL pulses based on detection of the specimen within user-defined regions of interest. The software can be used through the command line or through a built-in graphical user interface. The live feed can be provided by a USB or Raspberry Pi camera.
Find more information here.
Manual for Pyper.
November 8th, 2017
Jumpei Matsumoto has submitted the following to OpenBehavior regarding 3D tracker, a 3D video tracking system for animal behavior.
3DTracker-FAB is an open source software for 3D-video based markerless computerized behavioral analysis for laboratory animals (currently mice and rats). The software uses multiple depth cameras to reconstruct full 3D images of animals and fit skeletal models to the 3D image to estimate 3D pose of the animals.
More information on 3D tracker may be found on the system’s website, www.3dtracker.org
Additionally, a dynamic poster on the system was presented on November 12, 2017 at the Society for Neuroscience annual meeting. Click here for more information.
November 3, 2017
Greg Silas, from the University of Ottawa, has kindly contributed the following to OpenBehavior.
“Silasi et al developed a low-cost system for fully autonomous training of group housed mice on a forelimb motor task. We demonstrate the feasibility of tracking both end-point as well as kinematic performance of individual mice, each performing thousands of trials over 2.5 months. The task is run and controlled by a Raspberry Pi microcomputer, which allows for cages to be monitored remotely through an active internet connection.”
The DropBox folder containing the python code may be found here.
Silasi, G., Boyd, J., Bolanos, F., LeDue, J., Scott, S. H., & Murphy, T. H. (2017). Individualized tracking of self-directed motor learning in group-housed mice performing a skilled lever positioning task in the home cage. Journal of Neurophysiology, jn.00115.2017. https://doi.org/10.1152/jn.00115.2017
Click here to submit a piece of open-source software or hardware to OpenBehavior.
October 31, 2016
Mousetrap, an open-source software plugin to record and analyze mouse movements in computerized lab experiments, was developed by Pascal Kieslich and Felix Henninger, both located in Germany.
Mousetrap is a plugin that is used with OpenSesame software for mouse-tracking, or the analysis of mouse movements during computerized lab experiments which can serve as an indicator of commitment or conflict in decision making. The integration of Mousetrap with a general-purpose graphical experiment builder also allows users to access other core features and software extensions of OpenSesame, which offers more flexibility to users when designing experiments. Mousetrap is available for use across all platforms (Linux, Windows and Mac) and the data collected with the software can also be imported directly into R for analysis with an available Mousetrap package.
The GitHub for this project may be found here.