In a recent Behavior Research Methods article, Soaleha Shams and colleagues share Argus, a data extraction and analysis tool built in the open-source R language for tracking zebrafish behavior.
Based on a formerly developed custom-software for zebrafish behavior tracking, Argus was developed with behavioral researchers in mind. It includes a new, user-friendly, and efficient graphical user interface and offers simplicity and flexibility in measuring complex zebrafish behavior through customizable parameters set by the researcher. The program is validated against two commercially available programs for zebrafish behavior analysis, and measures up in its ability to track speed, freezing, erratic movement, and interindividual distance. In summary, Argus is shown to be a novel, cost- effective, and customizable method for the analysis and quantification of both single and socially interacting zebrafish.