In Current Protocols in Neuroscience, Alexander Jacob and colleagues share their open source compact head-mounted endoscope (CHEndoscope) for imaging in the awake behaving mouse.
This miniature microscope device is designed to provide an accessible set of calcium imaging tools to investigate the relationship between behavior and population neuronal activity for in vivo rodents. The CHEndoscope is open source, flexible, and consists of only 4 plastic components that can be 3D printed. It uses an implanted gradient index (GRIN) lens in conjunction with the genetically encoded calcium indicator GCaMP6 to image calcium transients from hundreds of neurons simultaneously in awake behaving mice. The aim of the open source model is to provide an accessible and flexible set of calcium imaging tools for the neuroscience research community. The linked article describes in depth the assembly, surgical implantation, data collection, and processing of calcium signals using the CHEndoscope.
This research tool was created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the tool was described, and include an RRID in the Materials and Methods of your future publications. Project portal RRID:SCR_021587; Software RRID:SCR_021616
Link to paper: https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/cpns.51
Jacob, A. D., Ramsaran, A. I., Mocle, A. J., Tran, L. M., Yan, C., Frankland, P. W., & Josselyn, S. A. (2018). A compact head‐mounted endoscope for in vivo calcium imaging in freely behaving mice. Current Protocols in Neuroscience, 84, e51. doi: 10.1002/cpns.51
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