Annalisa Scimemi, of the Department of Biology at SUNY Albany, has shared the following Python based code to track movement of labelled paws in grooming and freely behaving mice in an article published by PLoS Computational Biology.
Traditional approaches to analyze grooming rely on manually scoring the time of onset and duration of each grooming episode. This type of analysis is time-consuming and provides limited information about finer aspects of grooming behaviors, which are important to understand bilateral coordination in mice. Currently available commercial and freeware video-tracking software allow automated tracking of the whole body of a mouse or of its head and tail, not of individual paws. M-Track is an open-source code that allows users to simultaneously track the movement of individual paws during spontaneous grooming episodes and walking in multiple freely-behaving mice/rats. This toolbox provides a simple platform to perform trajectory analysis of paw movement. M-Track provides a valuable and user-friendly interface to streamline the analysis of spontaneous grooming in biomedical research