Multi-channel Fiber Photometry

Qingchun Guo and colleagues share their cost-effective, multi-channel fiber photometry system in Biomedical Optics Express.

Fiber photometry is a viable tool for recording in vivo calcium activity in freely behaving animals. In combination with genetically encoded calcium indicators, this tool can be used to measure neuronal and population activity from a genetically defined subset of neurons. Guo and colleagues have developed a set-up to allow for recording from multiple brain regions, or multiple animals, simultaneously with the use of a galvano-mirror system. This creative and simple solution reduces the number of detectors necessary for multi-channel data collection. This expands the ability of researchers to collect calcium imaging data from many subjects in a cost-effective way.