3D Printed Multi Pump System
Each pump uses an Arduino Nano V3 in order to run a stepper motor, which is used to dispense the liquid slowly, and with extreme precision (0.5μL for a 10mL syringe). The stepper is run on the Acellstepper library and uses Java C++ language. A general code is used for each pump, and command line inputs are needed to specify the number of steps, speed, and direction (dispensing or taking up liquid) of each pump. The entire pump rack can also be controlled simultaneously using a USB Hub.
An incredibly detailed protocol to build and implement this multipump system is available on Instructables.com!
This research tool was created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the tool was described, and include an RRID in the Materials and Methods of your future publications. RRID:SCR_023575
Special thanks to Hannah Kibler, an undergraduate neuroscience major, for providing this project summary! This summary is part of a collection from students in a Computational Methods for Neuroscience Course at American University.